Prajnaparamita Dhar

School of Engineering - Chemical & Petroleum Engineering
Associate Professor
Primary office:
785.864.4969
Learned Hall, 4132
University of Kansas
1530 West 15th Street
Lawrence, KS 66045


Summary

Education

Research

The primary overarching goals of the PI's research are to understand nature's rules that govern biological self-assembled processes. In particular, the PI is interested in correlating mechanical and structural properties of self-assembled biological processes that ultimately ensure proper functioning of biological systems. At the same time she is interested in developing interdisciplinary collaborations to study interesting problems in biology as well as training the next generation of scientists with interdisciplinary interests. For the past 8 years the PI has been studying interfacial phenomenon in both synthetic and biological amphiphilic molecules using both experimental techniques and theoretical analysis to understand the dynamics of self-assembly at interfaces and more recently in bulk systems. Additionally, the PI has had experience developing two different microrheology techniques (passive and active microrhelogy), both of which have been cited heavily. The PI has had more than five years of experience working with replacement lung surfactants, understanding and relating the role of lipid-protein interactions on structural and mechanical properties of lung surfactants using experimental and theoretical techniques developed by her, as well as guiding graduate and undergraduate student research projects. Her research efforts till date has been heavily cited, and was also recently featured in the NIH Directors blog.

Research Interests

  • Lipid-protein interactions
  • Surfactants at Interfaces
  • Microrheology
  • Microscopy
  • Protein aggregation at surfaces and interfaces
  • Microfluidics

Selected Publications

Antunez, Lorena, Andrea Livin``````````good, Cory Berland, and Prajna Dhar. “‘Physicochemical Properties of Aluminum Adjuvants Modulate Reorganization of Phospholipid Domains in Model Membranes.’” Journal Articles. Molecular Pharmaceutics 13, no. 5 (February 2016): 1731–1737.
Chakraborty, Aishik, Nicolas Mucci, M L Tan, A Steckly, T Zheng, Laird Forrest, and Prajna Dhar. “Phospholipid Composition Modulates Carbon Nanodiamond Induced Alterations in Phospholipid Domain Formation.” Journal Articles. Langmuir 31 (April 2015): 5093–5104.
Sprouse, Patricia, Neal T Dittmer, K J Kramer, M Kanost, Prajna Dhar, and Stevin Gehrke. “Characterization of the Secondary Structure of CP30, a Highly Repetitive Ampholytic Protein in Beetle Elytral Cuticle.” Journal Articles. Macromolecular Symposia, 2015.
El-Gendy, N. A., A. Kaviratna, C. J. Berkland, and P. Dhar. “Delivery and Performance of Surfactant Replacement Therapies to Treat Pulmonary Disorders.” Journal Articles. Therapeutic Delivery 4, no. 8 (2013).

Selected Presentations

Dhar, P. (1/1/2015 - 12/31/2015). Lateral Clustering in Lipid and Protein films. University of Minnesota
Chakraborty, A., & Dhar, P. (11/30/2014). Carbon Nanodiamond Induced Alterations in Phospholipid Domain Formation Depends on Lipid Saturation and Headgroup Charge
Ghazvini, S., & Dhar, P. (12/31/2014). 5. Active Microrheology: A tool to monitor phases and phase transitions in phosphatidylethanolamine. ACS Colloids Annual Meeting 2015
Mucci, N., Gamblin, T. C., & Dhar, P. (12/31/2014). Monitoring Adsorption and Aggregation of an Intrinsically Disordered Protein at Solid Substrates. AIChE Annual Meeting 2014. http://www3.aiche.org/proceedings/AuthorDetails.aspx?PersonID=168564

Selected Grants

Morphogenetic Tissue Movements in Early Embryos. $1158344.00 (55119 (Sub-award amount)). (9/15/2015 - 7/31/2018). Federal. Status: Funded. Collaborator
Preliminary studies of lung surfactant lipid nanoparticle interactions. $30000.00. (2/2/2014 - 6/30/2014). Federal. Status: Funded
Preliminary studies of lung surfactant lipid nanoparticle interactions. $30000.00. (2/2/2014 - 6/30/2014). Federal. Status: Funded
Lipid membrane-protein interactions during the early stages of Tau protein aggregation. $275000.00. Submitted 6/1/2014 (12/31/2014). Federal. Status: Not Funded

Selected Awards & Honors

KU Women of Distinction
Emily Taylor Center, KU

Honors

  • American Lung Association Senior Research Training Fellowship, 2010 (declined due to change of status from Post-doctoral Researcher to Assistant Professor in August 2010)
  • Travel Award to the Gordon Research Conference in Soft Condensed Matter, August 2009
  • Recipient of Russell and Dorothy Johnsen Dissertation Award, 2007-2008
  • Travel Award to the Gordon Research Conference on Physics and Chemistry of Microfluidics, 2007

Research Interests

Molecular Engineering and Interfacial Nanomedicine Lab

Biological self-assembly processes involve lipid-protein interactions that influence a wide variety of cellular processes (e.g., signal transduction, intracellular transport, antimicrobial defense, and energy conversion). Nanoscale fluctuations in lipid bilayers, that form the structural basis of the cell membrane, can influence the protein structure and dynamics during health as well as during the onset and progression of disease. In fact, a wide spectrum of diseases is a result of abnormal or deficient lipid-protein interactions. For example, recent experimental evidence implicates lipid bilayers in the aggregation of proteins that results in the formation of amyloid plaques or filamentous structures. These structures form a common pathology of degenerative disorders affecting the central nervous system (e.g., Alzheimer's disease,) and a variety of peripheral tissues (e.g., type II diabetes). Understanding nature's rules for biological self assembly is crucial to achieve highly specific medical intervention at the molecular scale by developing smart nanomaterials that can detect, cure, or replace diseased cells and tissues (the primary goals of nanomedicine). A major challenge in nanomedicine is to develop a thorough understanding of the physical and chemical properties of self-assembled biological structures at the molecular and cellular level. Additionally, smarter strategies are required for engineering miniature devices that work synergistically within the human body to provide more efficient medical therapies.

Lipid Protein Interactions

The long term research goals of my laboratory are to advance the field of Nanomedicine, by employing a suite of biophysical techniques to (i) develop a thorough understanding of the organization of the cellular architecture at the molecular and cellular level, and (ii) engineer miniature and efficient drug-delivery devices that work synergistically within the human body. Reorganizations in the molecular and/or cellular architecture may lead to the onset and progression of a wide spectrum of diseases including neurodegenerative diseases such as Alzheimer's, respiratory diseases, and metastasis(spreading of cancer). Our lab is involved in understanding how abnormal or deficient lipid-protein interactions can alter the lateral organization of lipid molecules in cell membranes leading to the onset of various diseases. Using our unique active microrheology technique coupled with microscopy techniques, we will sensitively monitor small changes in the lateral organization of biological self-assembled systems (lipid membranes and cells) as a way to explore lipid-protein and protein-protein interactions and protein aggregation. Understanding the early stages of disease progression will open up new ways to detect and treat unhealthy cells. At the same time, current trends in medical research require an interdisciplinary team of scientists and engineers to work synergistically towards a common goal. Our lab provides training opportunities for this next generation of researchers.


Upcoming Events and Deadlines
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  • November 18th - AIChE Virtual Reception | 3pm - 5pm PST | Event Flyer | Zoom details

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